NRAMM releases Appion 2.1

The National Resource for Automated Molecular Microscopy, Sponsored by the National Center for Research Resources, Announces the Release of Appion 2.1
Added by Amber Herold about 13 years ago

Appion1 is a "pipeline" for processing and analysis of EM images. Appion is integrated with Leginon2 data acquisition but can also be used stand-alone after uploading images (either digital or scanned micrographs), or particle stacks, using tools provided as part of the package. Appion consists of a web based user interface linked to a set of python scripts that control several underlying integrated processing packages. All data input and output and communication between packages is managed using tightly integrated SQL databases. The goal is to have all control of the processing pipeline managed from a web based user interface and all output from the processing presented using web based viewing tools.

Appion provides wrappers for a variety of software including: EMAN3, Spider4, Frealign5, Imagic6, XMIPP7, IMOD8, ProTomo9, ACE10, CTFFind and CTFTilt11, findEM12, DogPicker13, TiltPicker14, RMeasure5, EM-BFACTOR15, and Chimera16. These packages are not part of the distribution and must be downloaded separately under their own license agreements. These packages must also be acknowledged by appropriate citations when used within Appion. Appropriate citations are provided on the individual pages in Appion as well as at:

Appion is open source software released under the Apache License, Version 2.0, and is available for download at:

If you download Appion we strongly encourage you to register as an Appion user at: This will allow us to keep you informed of new releases, bug fixes, and other useful information, and also allow us to keep track of the user base which is important to ensure future support of the software.

Please contact us at to report any errors or omissions.